	   SnapGene      œGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCgccaccatggcgttccggcaggcgctgcagctggcggcctgcgggctggccgggggctcggccgccgtgctcttctcggccgtggcggtagggaagccgcgcgcaggcggggacgcggagccacgcccggctgagccgccggcctgggcggggggcgcgcggccgggccccggtgtctgggaccccaactgggacaggcgagaaccactgtctctgatcaacgtgcggaagaggaacgtggaatctggggaagaagagctggcgtccaagctggaccactacaaagccaaggccacgcggcacatcttcctcatcaggcattcccagtaccacgtggatggctccctggagaaggaccgcactctgaccccgctgggtcgggagcaggctgaactcactgggctccgcctggcaagcttggggttgaagtttaataaaatcgtccattcgtctatgacgcgcgccatagagaccaccgatatcatcagccggcacctgccaggcgtctgcaaagtcagcacagatctgctgcgggaaggcgcccccatcgagccagacccgcccgtgtctcattggaagccggaagctgtgcagtattacgaagacggagcccggatcgaggccgccttccggaactacatccaccgcgcagatgccaggcaggaggaggacagttacgagatcttcatctgtcacgccaacgtcatccgctacatcgtgtgcagcatcccgccgctgttgtccgctggggattttgtgcttctggggtccCTCGAGTACCCATACGACGTACCTGATTATGCAACTTATCCTTATGATGTACCTGATTATGCTAGCTATCCTTATGATGTCCCAGACTACGCATAGTGAGGGCCCGATATCTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAAACGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCAGATCTGCGCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGG  ;9   $   $      #      &   !                  -      0   &               &                     !               "                                 !               )         *   )            5      #   0   (   '      ,         	   +                                 !                      3                                                                        !   #                                                      "   !      *            #                     &   $                                                                                  +   &   !          ,                            &                      %   %         *                           #      +   ;   $         $                        %               .   "      ,      	               (   "      $      $   #         =   %          #   &      #      $   *      0        $ Y áîï'PQR±^¦·&Ž·‡ˆù-	€	¥	®	¯	S
,}%è%©Ç4¶j¦¸$ á xâ b(my²6Ï§F¸ñ‰$-Æ	Œª5·´�_þi6ê Z ÷ 1_¸Ýäúa	°	Ù	a
“
€+{&Èk~¹#  ÈNåðLS_J!'�œp	.	n	�	¦	T
¦
F~Ú&éÂxJüc¿§> t û c/7QTßŠ Ï
�©Ç/·6`ëF& ´�£ª³Ðù 2i¹�œ¬°÷
o†Î»Q±Âçmµê{»ÿ˜7G! Îãž
 ¨=}æd%.Üîô+·Ð!d˜«¸šsJ�«j·‘å â y!)nzËWG‰òå
¶
Ù„��O¾ ø ¬_±f ®Þb	Ú	ß	”
�É,¢N|É>ä�£D  Œ MR’Y±	Üˆ�×-Gl‚ÉöW ´Õ`Ì"ûâ	b
š
º
=†'tâ'>¥ l¡àº [ f2¹å 	Âssêl—óÐ® I-  ÉOVÃÏÛ$MT¿°Ôûf‚	È	Ö	Ûi4�¸»h;K�š A`½Š|H^¨? ]‡ºËå÷U¥Mó(�Á	…	U
w
žæG¼'_<æ#r0 œ {æñ;=K¬�•Î5�	o	§	:
’ÚU ²4<WpÄÍ[lêwœÃy”{ý±d™\w&   “ TÞêöG[`Ý'"ç*=q/	h	‰	§
5rx¬#ÑXs)×âô¦pÀË d¡%RUÝ‹pv›žªò2º9<€aG ‚�a¡"	è0JS7�‰9Çì u ü Áà_Ð
¸Ó<–ñé#OP t˜0?º8àHˆÈ§«à²b¬×„Ï|Ä�[& ž´·dÑúº�­e	¢ê“ÿ¼Ý¯²n¶ž»ÚSÌ¼Ë Á q‹ M‚•‡ÏLšR.pˆ	ÃDëB€™«H¨Î µ«ÐbpªE¾€$°;8P�Ž „¤A3j±ø
®øÇè	|�  ¯Fë	—
ï`_¸Ñ7™Ó % Ï˜'~	³
¦¹Y›QZ�k’ 7 ä©Öô>Qçh
Ž
&/MeëU[t7‘Ÿ ! Ÿ’Å!ce˜	Ýâõ,zËÔ"œ¬ KV^¬¸!vˆ¶¸Éíæîö ¹ Å î AJûE*‚ÍÀó\¾·
É
·×óÚ?Ž/qÜâ 1 {X/Š££ÌêP‘KL¿øB€ > ã zÚ"UHæ
YBmePQA   « poÌ(‘æ$	ñ	
•™�-…Ç37VŠ‹K|" ù Ì`¯²¨{åùþ¬Ý	à	¤
ã
m„Ì-ËOq%?»¡¤yÎB ­Kc	ï	•
™+¤†ÕÍ! #Y­gïˆ
¯ÚßÛ	tv‚Êi}„¼åLEÒ@?A ý™Š¯n
£í ÓÊyŽw•ŒáÓU SÃ	¡
–Ôç‡÷NR M � Õ N2k¾ží.Hmë	ÄÖ È þ %•Z”¨Á²	¥íÎPÝ<LE‰ØƒÃÊáXB¡.  3j‹¾[“ÃÐî±% . aœhÍâì#™±ÜáÌ
ö™u"ìúó:ø*-’ºÈQÄMC   C ~ \ã	>|ãŽ¦áŠü�¢Ôá€%  µÖ‚gé>	»
ù…Ï(}?c!)ÏS
! c w Ì Æíü]	3
c
›
Ø
í
>k‡Þý(ºw…àî˜/mŸÏ» /3´aæ	}	È(StëIPßJ \ ¼ ñ D›þ¼nyï8D
x¾t�ù4t8˜G½ÿ1a ígiŒ|³ñÓIÃH7mô&í† ¨ÂtŸºÃö	Ò
‹Óž,¢>Ñ¯) ²ÓPÜã%-NY›ãÍgï(;÷ƒ	Æ	Ô	×	Âgj‚¹§2<†›‹ŸF�  €`˜©5¼,=‚B~‡}I©@Y  , | ÊW€ÄÀ±üG<	í	Ü—iêCøa¹_*  ÐUW…‡ÕAí·�	¼	É	Ò	è	


x€Àåïó¥ï0Lm{¾"Ð=Æ#tÀW) ˆ»qÌÕ!:	CNŒt	†	•	Ÿ	ê	›Ÿãçmz³½ç(ðÖ¿`cy14?ÈÏç~ HV·ÜôÂ©Âñ
¹bïÑÜ-|©Ö%—$.]s ^ø�)Ë	V
®²öúþp¢âõõµ!=ïõt ‡ æ!¦ÀžÙ	x
H­êãÂ5  � ÒØç<¼È.>Ê™£ƒÐƒ†–½Ï/		¢	JVXqÎê\mx¬Ä•´zj|ýÑùþ×yÁM ¡|€úþv›þŽ¹õ	Š5=Å€‰�^Ù�²6h#  ŽZ±†¡­O
;
p
¡³Ê'v>\´ËÕzÙdüV“Àe];x”0 všòÿLk<‘Ò6p	¨	
I
“ÛÀ[ïAëå•ñN^o�2q}ËòÒút’šLg¦TŽ4( ë÷ú	AD\¢ý(–s2r7
sy$cf"G¿õBYtâd‘®õ�¸ÁûÌ+' ! Ú .»2ØÞ‰¬âIs.aß0	w	�	þ	.
¨
ÒñK*ØãYfÝ´0vÕ* ˜ YßHQ+ŽvzÖ+>Ou}6|‚­:úgÃÌ¦$§=â, j ” ¤ ² 7Ue=7a×ë#èU8	i	Š	¾	

®
Â
$-9CN‚¥‡BÁøqZ à  �	m	­	`
’
#|çµŽb¾5=}· ¢PSVÞŸ¯œíóµ*Ï �ÁæNr½Iª¶	 eÈs=Öé�H+  UÎ#~Üö<¯Ó&LòŒg	ˆ	‘�ÙåqwŸ«±3»:™Öáó_‰;{"qv s ¹Žoç1ß±a8;{•ð ƒ¶Ï¡é’K¯ÆºARÊÀ:€§� $ ÄEbP—	TX6Už·PŸÈí �å[¢Ë#	¶
éO”KŠ "¬m
£
â
˜»Ô xvÍÑ?‹ÒT L Ô ý 2iŠ½ Â¤ì•OÂàêÃ$-Q B b v f[á\	{=¹!ß—àòÎû9€ÇPÎ$ šì§»`Ñ
Š½Ò7=ì0 ÒÅ	Ñ	ì	
	
w¿—¨–ò<¤…¸}ÅžŽ p9¿Ž”	š­Áâõ	¬áîô´ÐÛ bx03¨é}ôs\! u�™×»ÇYý;¼¡	H
I‰¿Éé³Ø]cyØŽpøùf¥g i 1@×¡áüv	¾ô­ÂeÜ³·) ÛÒž®f	"
r˜Õàò¼H�©½zµ!4<pu Ÿµ¸OÊæ
“Þ°ÅWJïQTŒ !1ëeº[	‰£Ñë”Nº6ŸÍßÌÂÆ/ŠÑ  oŒÆÖXû»Ð	G
¬ô¾ –½;í
³Áo·÷Û/�r(f Õ¹NÉˆ«Ðó½Mqì	¹À—ÄÔßñVîG¨Þœ‰3Ð3 *nDÇÈÔ…
†
‡
ABCUV»¼SÓÞïð()ìíCDEF�¦§ÆÇ™š›ˆš¬&012©ªÏ_ � P+JoEÅN
DWT/èü‰*ÁÈÔ'I«³` 	†ƒ–Õóˆ
ò
›0
E‚í®›­¯ � Â ô GQÅ,K‹Eq«dU¿0ß9Ð!ÈÉ~(Jž I ¿ Ý k¦ÄÑ3	ß
Féñ «<dn9Qh¬´ S ¶F¦Xcg¹]ýŠ›æÂôóaƒ O ¬p2ÆO
S�m%±+ÕÙ	� ¨ × –¥vö„Öôz	]-¼}'sœ 4—ù
ü
RLœ¥Èo	°m … 
7‡uB4Þbk‘‰
ó
3¯ùÊ1Ü0«ƒîTØ‚®é …6²ÄFéF
B¯+Ø r ‘ õ RvªÆLÁªÌ˜
SY¹2LVÀàqZÞäþ Ã Hn€éÛ¼Fð @¬Ò1nšÑ"›Êç†‹‰  Ê -ì
fyr:FË…�ÔKŸ ,c°GÚøŒ™§°ïøka`e8²ÉD) J g Ò Þ ÐŒ’	´
à
”òw7={µ" ƒ À mlÃÅÒñ Ýê·ºTR¬ñl:­ & n p *k™(ÐG§ãåi— ( É§	4	rN¨¡Zœ[‚eoRX“ 4 ; ¶ ë >øBöRž+^*	:©Ó‹çGõ3•nƒ„ 8 F T _ `—¤×õ?Í	�
dºãu8 Ãñk  åªG³i
'0NY^gÖVþÅ’*¡v™£Æb ·úx\§èhG�8‹fì\œbô4 žqÝ"…pP
«
öTÈŸ¤jß÷‚&,4V?·ç! P 'Â“ó›ž3–ãVÌäIŒN÷6Cn‹²#£Ö
¥w×’z# �­êÆdj*f™		´üÞ-7Õ#­>ÚÌL_­Ú"l¹î÷ï0�  :|‘ãÇ´	X
(ÒR{�‘©)W¹3Þè‰Ê÷  W Š Kß]d…š¿Ûu
¸
ØÚ£¯h]gEã © _Ë]¸ÕÛQªO~Xt º Æ ï B“ü—¦wÎ÷Áô{	1
Ÿ
Ê
Ö
ô¯ˆ(�Ý * Ø 5F+5áƒqt�×õ6	º	æ	^t@�.½0Ï;r�J |ÂY‹ÍëM­Lp“Àù9 5Ó0B¤¤ú
SJQæú™£&
±kn� 2 � l�Àè8ƒ�’¦áÉ„y•Ä ˜ø	ý
¡MµC v‰GV ’ô
C°üúnõb\iìüê ? ¤Ð5cZQ1¬·U † ä {ƒ#ˆßIú	Š
ËRïÙƒþ Û8#Cl	ç
ÿ
4³ 2Ýf“„BÚ'¯ÝTZ ¡ ••>©	ò	–†È4aPê—„–Lq¾} §¾†Í’1Å�“8\ÿi°, q¨Òð74âz
š<ƒGWžä³Ø ¬ óp)çÅ³	%	O	T	€
. ÅG‹CŒÙå" ö wÇMaKlx°0I]¥¶%›oã,	¤	¥
+*EzÁ$wÆIû! s ú Í³©Ê	ø<|ˆå€Î
ä
ö
n…Í¨.ºØPc—¶lz—E «‘°XËÿ!­Þ	á	™
<Má&rk¢Èõ–òÏCVßÿ* ’ › S\†¹zÂÚé¾T«æúÍ	TÌÐZ3Wr€›·gÿ@¼^¥o[Êå >ÜG‡šñÒÖÎ;œè"8ÆŒO @cL”d	©­þ®o‡#�ÙËOpœŠ 6 ®‘Õêó�
–
²
Lá_^yÓ¥›ÿ]€‡ì# 0 = Ÿ ¸ Ä í @IoÙúD'�T¢¿½ð	È
XA,Æ2dp÷@Û{ ®‰®Jzä	ÙÜ	lsuƒËìp~$ƒ…Ì ”>@  $Zí“ÀÂ	Í;K½Óæ†MAÕ  Ë ›g˜°Ûàìì
jüÎ|�„(÷,‘ÓÃLB .h‹ðmžÒî7³	ws��ø3Ç+G¡%F¼þ…  ±âšÌì:œ	äféîBl~z!Ï"EV Ô¶‹Û¨±ðùl¸²aoÕ,{Õ–îâ†&  þÉ°ùj…š Ï…Ñý.o
Zî@=«Ô³üÊ�Öx5S�3:“$ -d«H�-`y~Þ1Ž	ý	�b¤!F†9AJË÷�<ú/Y*Ô  ß T¬	_
‘
~°Õèˆ¶— �µ
`¹¶O¦ A K a Ó ™¦l
¢
á
¼— 8>#S h Ñ~ü“	Ä	
•ó\xwø¤| n‹Ï	!IŒ
Å;'e Ž O…mÄÆÓòM
@ë¸¿îUÀÅÓÝì­‡® H N Á jŠÞ
f\ÞlÏå Ÿóòm „ Ö 3a�Q\2Û»/nªS× q É +buÙè÷ex©Ú»¦îj?m„æYãCý ' o jlÂÏÜMSâä : E ÿ =–£[�]9fŒ7¨ÒFÄ)ðjm‚˜¢  &šâ )•³	ÑÇQH‹Þ =B�Š¨Ù(3UË¢¤ÝÖy/  ) V ‰ 4’\^Ês€ 5	å	t
sÙ©¢®:gW\f € kŒ¿Ò7‚A÷	å’´8ƒ”j ‚²û&ýSY ¦”ÄÑï¨	‚ŸO[–�ãþhƒp½ …¸�\÷$šnâ+	Í
*;§)š¶vfHÇô•ñUÉ–D / 5 < · ì ?ØùCbSŒ
Ç
x
:œþ\ÅNo›z 
�­I’¿ãí²ëë
ÌŽ÷ÆÍ#*Œ'ö+“»„= 
 iŸÎ,_}Ýü	·ã?@ªÔèíAû+;ù!.4RX…  ` ˜^
k
�
}»v£¥– G „Ši`‰Î	Ý
?P[eä‹¿ÄÒò†&d 9 D atØáçöÙeiÑÛA�§'T¡£ÜÕâlx� U  ˆ ¥]@rä	s
Øä•â9ýg‚ú¼ü 	 ·×	¬H‘mêÆ
)¦¶�)@Æ&*[” Tš ƒhqˆØ]
j
r
Ü
OZdh×Ð¾Ñ¢Û…ûw¤c+  ¶«p´G	¼
½
(_`abc£¤¥µ×‹Œ^†"#$%b“½Ðúbc& æ }æ%¯µAKQÇ?	H	

(
=
¾
1Øú_W‡ÿ~þd©Âc+ dÇ!  ¸yLLRý^	
4
¿
2Ùû9Œþí)X|¾x±áïç,«£Ð d g°îœ
g °0_cñe  ³]¶éú@	
)
Ù
iHˆ`ýj»ªÃ™dõ5 , x Í ç ~ç&Çíû.£BR¨È#CC	I	
$
>
d
î
?ŽlßÍ]ˆ�ÿRXe†n^¼È œô4“µ	bþ_	~	Q
3Ú:Uµ}'qy²âè¬¤è Þ#¿-	}
ñ�¥ÉkYT-Ñà 0rz†ç÷É&)Ñƒuwì5-J@NQ¸ß  Ÿ¹MqÔMSr	
,
5
¬
À
ü íÿàîø*¿\hðWK ôŸ½ð9Yy¿uÍå�Œ³9Ýv¦d¾ò{	 Q ½ ò EÃÿ”4E
·ÿä‘7•4ÆO¤×x)b³f  ] e (œñœôhz—±nï5WJh°4Dou™1`Ü 2�“V‰ ÝEo�
ú:Oø—ßó`‘$Í†±”HØ& xj�®%´Çk´:êû8	š	j…ß]Öa"IÍÔnõ#m61‘ �h}ª	K
þkZeÛ'î ëe^ÖAJA	
Ú
é
‰Ä¼?P8šü`®øV¡ºïø\ î�ò+·Ôg*

µýI.8$®«ÄÛMö±‡ð  y :ÈN/} C©De
!6@K mŸ|‰’S‡£*ßi³½ è èð'¡©Ãu;ÉÓ
ï
�ÓS^¡-ž ªô?4œåéË îäm¹¼È>Ä÷ D	
%
à0CÎž€Y�XºÒ¨°_% Î ü’»¤SY$ix	J	µ	 
?
Y
Œ•ÔÝ)Âçfs­DoŠÉòø6%  X àäí&O^5ÎE¶ð†Üø`		Ø	R
ÅÙ$‹¨3³i^ýh¥é �.œÿ1hc›«Û¶ƒ~°™éºF�ä ‹ L³ÔQ^eÿ¹
…¡ÁÛ=r‡œ¤ã,FŒk ­* ÊÝõ:FZä¤e”
)4<À„	Ç	Õ	9
v
4";VoÃhk~vº“(z€°˜G] —�ß$`À^!	u¹I¦ªR«ƒˆÃ~NZ a™Ü÷D½-CªÍ  —&	g
Ê6êZ YuµÒõ ª Ì.~
˜ÖòŽl6>U.J‰PJAá ËXü˜§î‡øý«î	hÊ*£ÒxºKAà Ç ”1Ï§ 
°jDì
  - } �Åëè=	2
Ë
×
øÝõ˜„ëvùbí‰.)R¹ž	 » ð CýÁs{²xÂõH|	C
'R6HO`Þ#  + { ,VXâ„†@î'F¶ö;	»	Ó	ç	_Áî+¦/1÷<†s¿X G  BØs	ž	Ê	é	ýyæ¡ïô¾>Ç$Î-Á+   Ñ-˜¢u‚N‚¸ô	

&u[ˆÊä”ðûMU]in1|’¿ÐñÑs‘KÀL; — £ ± Ù 6X<ºù6CPˆÖê*r•ruÕNTt|7	½	

-
6
­
Á
#,8BM{e�ð áùAcf�À¥#Xáu$ Í"}õ;®KOñŒ	l	‡	�œÇØäì{´)ÎæŽ´ÀåM^q:| # rÃäDZÎ–	 èn‘N®:z”5@žÉ¿ h‰¼«ÿ
Zz<ÞÁßwúuëÐÍ
,$ Ö ¾�:u	 	ë	 
™Àáv¾èóñ£×�Úad2§²¶eèó  0êÅÚWZ	F
å	’•	:5ö.Py.´ot )IÔ¸C„
ñ
Tªòºçû/–ÒÝ'¥˜%/²^ R ¾ Ü ó FÄ 1¥¥Ã2	¸ Rcð8šÇØ}ªcg‚ § 6t•uÝ5õy	û
ÃKEÉ¤,A×&r*èl m©À˜Ë¯÷RÿX1K±EpÊš…�ÝŠˆ f m ‚ Ñ )‹Èð‘	q“v§¶
6ðzW–! 3 ^ µ ê _÷Aùwõ²*)	Ì	FÕŠâ2a3”uÅ% Ž�Áéò�Ü9i{„�oW
ª
³û'6Už£ãiöMö5"2kç>‘ö Þ4àpc™Ú¹	0
ž
Õ
Ô®PÎN‡�ID çÁIù˜ ¢à¬%xÃ £ˆÏ"FŸ	ù	þ
ûôa’[h¶ÙëÜ ò½”§=03á	N	S	y
;’`Ä²•ä×. š [yÙèJkw¯½É/HS¤µ;WÊ ‡ä
	£	õ
DÏ×by#–µÅþkú]jn" ž =�&?Ôò¡¾“±
KW†à^¨ý]­ÅcØoö?7ëÚN ŠlšÑ6—Ø	Á	krœë2ûoJ{‚ƒ Òÿ$ýÂ,  ÓáªµÈË„x„Ðëü-09�	›	€Y±En…<Iök}Ž ÎÕzÔí29’ @ }¥û«	
¯[_¸µ	 �L
1[ÎÚ©Ü <¢i~ÿdwœ\º8‹6>ÆŠÿlR2(ïü �‘Ñ6�ÿö	�ž‘­³fV7i „�[¾|û	‹
ê
Ë(â>wÅ?µ	Ìe9›ýðW<y ‡ s‰àæ_Ú@”&Sfù»•( å |$o‡�®×@JPÆlF	

'
<
\
q
Å
Û
¢´Š]Öý¨ÁZa¼Ú„ùÿa"  ì-f²¢ù"BKQB	
#
Ìüi{½QW°æ¯^ª¢ð] ›ó¾,’Óq	+
|
ð´ì%Ð[gpvMÞ$ ÜðDómX ¶þ9¯3–Þò_�”3ÅÌÜNu…“Û(�²Uˆ w Ž$9Õ7@	©	J
è
~„\!YO~Óû÷U°[$ ï9Ml¸»=Xhw
”Ü 5JÁ/Bæ r€¬óC›äh§²ñ5# Éì9E]d£þ0D3ª8
Ä!n„ #}ÀuŠ’¯˜+E—¹üj\ ––% 	f
t—HöCé5IˆÂ´MÌô~ û
í¦êªB
g¢QëG	= z – ¢ ° W;øB,Oé)?~”—¡t�&Es{µ:	ó	
"+7ALz%dZ‡Éãbe“úTö‘¤…àÍr¿,¾% " gªþ­ãJ	k	ÿ	�˜Æ×à;�ò¢Ùä]Þt�±µêÌ+ ¦ Û /é(sÄÙt1	Y	x	ƒ
ð
”9DÈ.+õ@ q-ç  l ´ Ð é ¨ö@¿vŠÛÇÊ(	�	©
&EQÔT‰0L	5jæ1Ž# ñ¢¼3‡Îß“</2bà	M	R	¸	/
Ô
Ó‘_ÃŸ«ZgŽwÛÖ& ™ ZàIR©ÓñÉ5w×ã,~Œ	À	°
›§1D„;H�Äj‚�Ÿ>ÓÙ8 ;€[
70®Í¨´û# r,ån�½?{Åø!PkE	
"
&
[
Ä
á=ûh§¯%åY»©` ï½8ºW?Wv{
}ƒÏŸXZuã±$ • ¯ V8c+èì“$¥é€%z´9	
A
*Ãƒ¡èùHDF[Ëó* k ¥ ³ Ï fõ?rýÚÆ		'	L	Q	X	j	·	‚
Ž—Öß%:DPˆ/CÂÇs�p�Úæ :¼VZVj‹	¿	
!
Z
¯
Ã
/¦itŒ®ú70 ­ ® 89ô>bq*ØÙTUy	&	K	P	U	V	W	¶	@
�
�–ÕÞ.OÆghEr‹ŒÊù  1˜  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                       0                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       	            
                                           	                                                                                   !                                                                                                                         5   	                                        	                                                                                                                 	                              	                     <   "         
                                                            	                           	                      #   =   )      !   *      !   &   #      ‹   U         n   u               X                                                                                                                                                                            0                                                                                                                                                                                                                                                                                           	    
                 	                             !                                             5 	              	                                       	          	       < "   
                    	         	        # = )  ! *  ! & #  ‹ U   n u     X   sÉ  tû  u  uU  yù  ?  ‡�É
•/ž
k I]dq)ê€Å!,~	&	X	Y	x	¿	Lg‡¢+Ùë‘¨AÀŸçÏ
Í‡	d,ób™JÖ” U»¸ØvcïcïÏ
6Ì†	©ñ ” .U²Ê»ÌNQ·âüvzÖ2>¹†	Ÿ	É	Ë	õ	
.
©ñ$syqÅÜgmâ5BÃïC†ø4¸ªÄ½Š,A‹˜º,JA,A,˜,Ý©1>ÝD�×ä 	|Ð
Çh	âËÔæüò‚½Þ§<”ÿN·Ý¯ƒÅ_m¸ ˆ	Ýœ�bÚß³m¸Sc¬í	j	C‰m¸Û«¹ î AûÀóÜLSß/œ	ä"¨ƒG’$’¥Ôw
¢É
ã“„�'œéõZ"ÐWr(ÊµEWü`ý­Øð¹ î Aû¿°ÔÀó?Ž^Ü‰* É? ¤t˜ýí«Œáá¾Ã_!F dãñ®|ú_!œF£áÐžt˜ð_J!�n	¦	éPÐvÞÔI®ö²	P}š‘‘ávGCfý:sÓÁ	±Ò‘7ÏÔ
	
K½Äb—	í’ž/_`mnK¥Ô¸¹!° 	-	b
w
’Ú¢Éçp™lºµ ›93N²µ�"	‰’ž
®¨¿ì
IbÙbªÊ&		j	k	ŽÖQt®Ã#0Äiø/©Ÿ—*ib|*2D¢27
¿t‘–U…RÝ›¸õZ"—ß.£¢	xùŠõZ"rµJ¨¿ÊQ0 *t˜ðý_J!�œn	¦	F£í«éŒá™‰ÉL¨¿ÙÊQ®0 @O[ž·Ý¯” U|ÌØßãç!DU¼:V…¢úvÒ+2		8	p	†	þ	
7
›®ãöÎ©ëñ:>t‘ø‚Øý-´Y¨¿
bªÊ&Q0®öÉ’žÄbd—	Ü+!«JU«··Èí 5�oª
žöÏôëÃ&ƒ¾Ñ…f�:N!6@K£ßt˜˜'ý­µØ ›ð™áa2È„-Õµ°ø). SßŒX. Y›
Ò
ã
LSëáß 4/+:�ä‘ÍÖ)a�ç*O²8šüO™œ‰èò‚* ‡?aÌ
CD¢27
t‘Ö,—¡�& 	è¿sGÁó’¾Ó±Ö 	èSß‰¾39N²h!É•3K|þq¬iŠ½$zá	<›áßž dÜô+!«J«·åŸzœ V¸ˆÉöbœNeo›™ ZIR­JÉã~	k	�Æ×Ää]jíŒñÓ†�¡"	è‰ ÒÕÊŒƒƒÏ/t	Ÿ	ê	X½çmðÖj|`c1yç “ T_`nË¹!"çp 	.	h	‰	¦
„p¿Ð®ì8]cDÃÄƒ#ŠDü[¬b	”
 :±”nË¹ 	„Ð®ÕŒt	Ÿ	ê	½çðÖ`c1ç ÒÊƒƒÏ/Xmj|yÛ©
&TLjæ—
¸d¿u
¯ú	f ÒÕÜ÷AYãÊ(Œ–ƒsƒÏ(/;rt	ƒ	Ÿ	Æ	Ô	ê	y$Xc½ÂçgjmY¹âðdÖj|õ�`c1yÌçF+™ Zì8I]cR­JÉDã~	k	�Æ×ÃÄƒ#ŠÄä]jDü[�V
²ú¢âõõ!=õ¯F`_7vGVô
C\igªþ;ÞtêÌ+
î·Ð˜9{�W
'2çXÜu=†7Ná x (myFñ	Œº	‹ÓŠ K]‰Y¸
únÚP£êÀùü=X†Üu7N•«wuÐˆFŸûôa[hëE*‚?Ž/qÀ±G_• ¯ V+è“€%z´9	
*ùã%NÍï÷×	§2Ú«ç_Ð
æ
eë[€$e°<AŽÌ|	Öò'>R.HOÞáœ�%Ybe
…Úß]µ}9‡ènõ³¾6,ø!P"
ûh¯å©Ñ�­e	»¼ Lk<
“ÛÀAåqòšg¦4/7Ç~ Õ 2\nã	ï	ù+ã.máüG�Öž´·Ý¯S‹ëeAJA	
¼P®¡ï\_Ð
<Úæ
eAnï	ù+G«çeë[€$°ŽÜ÷AYã(–s(;rƒ	Æ	Ô	y$cÂgjY¹âdõ�ÌF+«ˆFŸûôa[hwuÐë¬n"Ê‰d g°îg0_šEã© + { ,VXâ„†@î'F¶ö;	»	Ó	ç	_Áî+¦/1÷<†s¿Xz – ¢ ° W;øB,Oé)?~”—¡t�&Es{µ:	ó	
"+7ALz%dZ‡Éãbe“úTö‘¤…àÍr¿,¾²ÓPÜã%-NY›ãÍgï(;÷ƒ	Æ	Ô	×	Âgj‚¹§2<†›‹ŸF� 3j‹¾[“ÃÐî±%u�™×»ÇYý;¼¡	H
I‰¿Éé³Ø]cyØŽpøùf¥g ÐUW…‡ÕAí·�	¼	É	Ò	è	


x€Àåïó¥ï0Lm{¾"Ð=Æ#tÀW@ }¥û«	
¯[_¸µŸ’Å!ce˜	Ýâõ,zËÔ"œ¬ KV^¬¸!vˆ¶¸Éíæîö  “ TÞêöG[`Ý'"ç*=q/	h	‰	§
5rx¬#ÑXs)×âô¦pÀËÉì9E]d£þ0D3ª8
Ä!n„ #}ÀuŠ’¯˜+E—¹üj\m©À˜Ë¯÷RÿX1K±EpÊš…�ÝŠˆ  i “ œ Tu{�™×Þæêñö1;@G[`»Ç×Ý=KY¡áü'ý"¬ç;�•¼Î*5=q�	/	h	o	v	‰	¡	§	:
H
§
5I’ÚUrx‰ ¬²¿É#4<WpÄÍÑé[l¾êôXswœ³Ãy”Ø]cy­ÂØ){Ž×âôpøýe¦±Üùdp™³·À\f¥Ë)gw' f m o q ‚ É Ñ )+bmu©jlÀ‹ÈÙè÷ex˜©ÂÚË»ð‘	¦¯î÷RjÏÿ?Xq1K“m±ÜEMpv§¶Ê
S„šâäæ6Y…�ÝãðCzWŠ–ý!ˆº Æ ï B“ü—¦wÎ÷Áô{	1
Ÿ
Ê
Ö
ô¯ˆ(�ÝÔ¶‹Û¨±ðùl¸²aoÕ,{Õ–îâ†" ™ ZgªÉàì9EI]dþR£©­ãþ0JÓñÉ5Dw×ã,3~ª	k	Œ	À	ÿ	8
°
�˜Æ×à;�Ä!n„› §#1D}„Àò;uHŠ’¢�¯ÄÙä]j‚�˜Ÿ+EÞ>t—�±µ¹Óêüj\ÌÙ+8š [yÉÍÙèì"9EJ]dkw}¯½Éõ/;HS£®þ0KO¤µ;WÊñ D‡Œä3ª	
	l	‡	£	8
õ
�œÇØäÄì!Dn{„ ´Ï#)}ÀÎ×æbuyŽ#Š’–µ¯Å´ÀåþM^k˜ú+E]jqn—¹ü:j\|S ¶F¦Xcg¹]ýŠ›æÂôóaƒï½8ºW?Wv{
}ƒÏŸXZuã±d¡%RUÝ‹pv›žªò2º9<€aGG „Ši`‰Î	Ý
?P[eä‹¿ÄÒò†&dº Æ ï Bq“üeu�™×—¦»ÇY¨wÎÒðý7÷4;¼Áâô{	¡	1
H
z
Ÿ
Ê
Ö
IÈ‰¿Éšéô<ƒ¯³GsØW]cyØŽ=pžÖéø�äùˆ³(f¥�ØÝHgÞ#¿-	}
ñ�¥ÉkYT-Ñà  +Õ     ÿ  ³         ³)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M¸92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄŸÙÇ'á¦5é¾˜b~c“›é†/Ñç6H@üfìuµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�\ C—…wuŸüÎaÃYUUÍÀŠÊŠÙ\À+ôî,gýñö€=g?2®¾«ýÿýJÀ…Ð;4õË ÃCøöMŒÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nåloê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+1X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»XíÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~±Á°»ñ¿æí'[Žá�6JSÒn;øèˆ BÎHÌMV¥9×PÔ:hØQn:  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "Ã<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5677" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4886-5677" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5678-5746" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2585-3379" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="843-1431" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4127-4715" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1747-2175" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="13" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2189-2518" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2369-2504" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3555-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5747-5851" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1497-1545" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3773-3779" color="#ffffff" type="standard"/><Segment range="3780-3797" color="#ffffff" type="standard"/><Segment name="-35" range="3798-3803" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3818-3839" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3725-3741" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3749-3765" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="733-759" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="763-789" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="793-819" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>133751de-e061-46cb-a9a0-62e6e87ef9e0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="9:28:1">2023.7.14</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  5        ¶         ¶)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M¸92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄUyfÑ�tW–ÒQ©U)jñ™!ñ›±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>p^ÜÖ|ó9‡eTtYW7(*+es ¬/Ð87¸±Ÿ÷ÄÚ õœüÊºú¯÷ýÿõ+?@ìÓ×,ãÙ4n3N î@	¿ÜÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨«š
UXN—Uj\Å¥® Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û�¸NkÑI!:ª>Cª5TïÀN‰›Ú¬†ÔGA„*Í†…Ô`hÜÐ®ðó  D9¬9‡€Ýtu°ã¸cÏ"¼8´Í ÏÂº¨µ‹Â?……ãAÄ4Ì9²qÀÊú3N#±Ê-áLàãš� Ðnø¶íú;ãÄT›Û`á�c8ÙÎ†ðçãö±÷ÔÅvÍ±17'°`SÂè±´nM´³=<^+=Ô36?1ì²Ø1�Ž�m‚X<ê¸Û ðà"9´“(9ü†háDè0Å€áó¯²:œ&Ï;ñ¬Ó6Ï®lúèq+®î ¬Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êíc·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜,{´�n;†<Ù)OI¸ïã¢"�8I#15Z”ç]CPè|u£aE¸é   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ! <?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1316" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="933" maxFusedRunOn="933" detectionMode="exactProteinMatch"><Segment range="1317-1328" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3752-3768" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3728-3744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3821-3842" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3776-3782" color="#ffffff" type="standard"/><Segment range="3783-3800" color="#ffffff" type="standard"/><Segment name="-35" range="3801-3806" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1500-1548" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5750-5854" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3558-3679" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2372-2507" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2192-2521" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1750-2178" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4130-4718" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="846-1434" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2588-3382" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5680" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4889-5680" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5681-5749" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="3xMyc" directionality="1" translationMW="3573.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="825" maxFusedRunOn="825" detectionMode="exactProteinMatch"><Segment range="733-822" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3 tandem Myc epitope tags&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ˆ<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="R1" sequence="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="731-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" meltingTemperature="69"><Component hybridizedRange="731-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC"/></BindingSite><BindingSite location="761-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTTGTTCC" meltingTemperature="62"><Component hybridizedRange="773-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATT"/><Component bases="AGTTTCTGTTCCAAATCTTCCTCTGATATT"/><Component hybridizedRange="768-772" bases="AGTTT"/><Component bases="T"/><Component hybridizedRange="761-766" bases="TGTTCC"/></BindingSite><BindingSite simplified="1" location="731-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" meltingTemperature="69"><Component hybridizedRange="731-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC"/></BindingSite><BindingSite simplified="1" location="761-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTTGTTCC" meltingTemperature="62"><Component hybridizedRange="773-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATT"/><Component bases="AGTTTCTGTTCCAAATCTTCCTCTGATATT"/><Component hybridizedRange="768-772" bases="AGTTT"/><Component bases="T"/><Component hybridizedRange="761-766" bases="TGTTCC"/></BindingSite></Primer><Primer recentID="0" name="F1" sequence="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="727-832" boundStrand="0" annealedBases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" meltingTemperature="71"><Component hybridizedRange="727-832" bases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC"/></BindingSite><BindingSite simplified="1" location="727-832" boundStrand="0" annealedBases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" meltingTemperature="71"><Component hybridizedRange="727-832" bases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC"/></BindingSite></Primer></Primers>
  ><Notes>
<UUID>554b079e-04cd-46e5-910a-3c8d9ddfc533</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="8:15:15">2020.4.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *T      ú  ž         ž)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M¸92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄuGØp†™Q¶±çPaÆI!ñ›±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>p^ÜÖ|ó9‡eTtYW7(*+es ¬/Ð87¸±Ÿ÷ÄÚ õœüÊºú¯÷ýÿõ+?@ìÓ×,ãÙ4n3N î@	¿ÜÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨«š
UXN—Uj\Å¥® Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û�¸NkÑI!:ª>Cª5TïÀN‰›Ú¬†ÔGA„*Í†…Ô`hÜÐ®ðó  D9¬9‡€Ýtu°ã¸cÏ"¼8´Í ÏÂº¨µ‹Â?……ãAÄ4Ì9²qÀÊú3N#±Ê-áLàãš� Ðnø¶íú;ãÄT›Û`á�c8ÙÎ†ðçãö±÷ÔÅvÍ±17'°`SÂè±´nM´³=<^+=Ô36?1ì²Ø1�Ž�m‚X<ê¸Û ðà"9´“(9ü†háDè0Å€áó¯²:œ&Ï;ñ¬Ó6Ï®lúèq+®î ¬Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êíc·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜,{´�n;†<Ù)OI¸ïã¢"�8I#15Z”ç]CPè|u£aE¸é   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !H<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5656" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4865-5656" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5657-5725" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2564-3358" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="822-1410" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4106-4694" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1726-2154" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2168-2497" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2348-2483" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3534-3655" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5726-5830" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="798" consecutiveTranslationNumbering="1" maxRunOn="801" maxFusedRunOn="801" detectionMode="exactProteinMatch"><Segment range="733-798" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1476-1524" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3752-3758" color="#ffffff" type="standard"/><Segment range="3759-3776" color="#ffffff" type="standard"/><Segment name="-35" range="3777-3782" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3797-3818" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3704-3720" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3728-3744" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1292" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="909" maxFusedRunOn="909" detectionMode="exactProteinMatch"><Segment range="1293-1304" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>70bcc161-158a-4f5f-9630-d4c4e88666d6</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="8:9:43">2020.4.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  
˜ý7zXZ  æÖ´F !   t/å£àa€
U] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x’ÉÛ5•ÀÌA#ËŸŒoS,X dSòèþ^¡EÖø –‹q¨¤£—µN‰þt‡í7’(CUŽ™LLNEË·Ãw£9?F\¹ûÃnÙ—
‚¤B=�Ñ(¤�¦<ø,vÁ®%0½Ñû‘`çíÂãü·1×™‚Õñ�ðVEçaŸI{::5_S¤b<<¬bËšÚ8v”÷AåÅŸn‰�p_TKaJg±~ƒ´)´ÆXãc¡\Â¶t2öãûPˆÃÁ€¸Px©R¶q�õ)ÌÎûrÌMQÈŒX…pòM}uÝM:) ìÆ§Ûóô5S`Žã£#pÅœ¶Ýß*Äü¬\æm`ÜzËYÎE†² ö¬¡H¼ØSA ô~1®H¹@Å}zJ
�=I�òßÅ
*Ç DÍðž¥[“ÓVa—_­·0ô9ƒÔ|Ùt{`Ê�®eº;uñOØÅ�4T7,ª$ˆVë�Bž+¹ÆRÐ˜=» T®æ�Œ>þ»î�'OÁº9œÄ¸AôËß´°™Q^�€ÝÖ"0H›À(­�®ˆ!Íß=^49_£ç¡$Íî{9ôðñ­óŽ½‚ú¬OÆ	ièwoXÜQT°ÄS;Ä¿¹hV›9Gö"ì_³&'JÚYüÊ6a½óßg\‰4-ýzDtEFºã©T†Ð¥zd»�¨Óí¼¥™VÝ`õÈOéÞÜ’é,®ž¬\˜	ŸÿÀ	€ÜOàû&êiÅ™/˜ŠøÍÝ�Ÿø=õÚîBý_´g˜ºáXO(ñÖÓ‡Õ‹tù4±{ñ<.¢w#‘sÐÒnê=Ë?\k;5ºÒ<Á“:÷A3‚ÛÆÝ|§'¾�çM”â~…•™?:ž%rÉ€Ö-	Ó÷ùÄ”vÙ“ô¦ÆÚ Ž›Û�¬Þ¬Í¿î£•…ï[Ôs/åŸñ ÇFè…ÉuÈö¾RÖª‘Ä±ø§”<Ê:e=úåþt‹Ÿâ[Åbô¡—¾5–láª-gaôBlt æmFäéýðàÔ×8�îÅ‰gPgYé{Zhi’Ñ²H�Xg‰ç7$æSG…ß7ÈÒa†iWŒ™#ÍèÿólaèýKíÛ@†¯|�Ù±‰Ý¶€úo« =fy± 	¸¦Å3¢YI
änA"å¼—V²ûuwÉõÄpá$$$3l5÷¡£–Gï§÷eTÃ™¡~ÜeNPý5JþÎÀtž¤lv,Oð^¢�Ñtðf3!N4=vEE�ŒYfâØ�ç¼^àÌ<böÏ—�tÖìÈ¸I˜³{ÙEüX+Aç¡Æ –¸õŽ‹Ñã=ˆSBT-iæJQÈG®_�	S6BÑº-¢¯Ftuã¨’ë)¦ü#zÊ/‚5œw{"âwM÷øëÐsÄØû·®Ð9Ráp^»�2ekóîQD©R˜Òž`¥lÃq4?ÃåC>äjü’Ž˜ª=éæFÄg[Q†—W dãÓGA½½P.åÅÏO±1‡ZY‹Á¨mŒÒê¨Ç£*»
³¢jâ5?—MëWÀ>Ð�@ö±ÎÔÃ™õ–*é¥¯Z…÷ÃÖõÉ^sq /â.$ÆÌ8„=xŸXJñ¨àh›�”÷Y‘ûfwÕ$Èà[oýöÕsN8XöâXuÏffO8/Å2nºwˆºý/=$*9	�çÆŒ@mšr~=/iÈd„ S­7›ñ-ºUagå?Ë-Rý¥ƒœß¶à"÷è%t½®ø²ð ±Ós•ÍöJü´¨î|µ£‡Å
lª¸[ †×ÈÙ*¼ËŠDMÀU®ª4ÁØê$j€îÁ_ã(+–�Ex0„ÍØÕã¥ŸÓl¿Öâ ìPÈ< á×œ£ms“?Ö¡ÊgÔÞŸ?«|÷^}Â�ÆÕ)ï»Å%þRhO‡­s�è¯ú8c%/òDÚ•—9uë¡•˜kþäËòpã{.(OÖ“ÖHH_ÁB)æË=G›‰Òì8NÄ†ÒäÂ{bûTBomiˆx&S®«ó¿~²Oo×>�aÕ‘Å®åSv³Ê¥±$'†bñUæps¿^4žÆeÖ¯ç«‰´G#ó1=7Úd-#ˆRÑO·–ÝNV8më¬"T×óÜ”íÇFåÃI5%¾úŠ¼Q4œ�õfÍbH.n8t$;Yû÷G“‰LÂ[—Ã°4BSKKš}éÕPÂFå0B­^gr'šãð½Ãüæ»~ÌNp®RÐ¦½NÕù/0”‡„z™Ê;¿Æ2èU3Àž‰ûd&;½û¡b^"%å|#å£ã+>‰¦$_Mr
_é€·¼Ò{¥B÷q’�ÌúúõœðJÁ/ ç:<ø1Ç¾¨žû‚$!õ`\æ/r[qYÉÇÑÀÒmâ“t2�ÕÚòN-¬Çý’�´tN$ò5¤Ðiî†j"”Ù·–-gb�ñ³¸> õªS® ãù§Œ¨8DÂÒFDâ<WZŸÁ6#nZ{Dg’V’k@×­`×�Êqü©d`.( 1Î(À)KRZ¼ëtçGÔ§7]¨ª¥wA›@ÿiü¯í’|x Í�óm°ÓcÂj¡÷¼C1dÿ…0Œ<Ÿ/mýìùÉÝ(ˆh¯„ƒ½ƒk—è.1[ëGwY}5~Ï
eÈÊÜi¶Ù«p mu{›Tq&	îî÷õŸ_�ÿŸHU­´šÍè°ˆX¢Úü¥°[Š oåUé"&n.œ¾FºìQ·î ryŒ†1år‡$Ä7øÂ>@M¨ÞŸ†Ê²:K_®[ügBÎ‘­ìEÂ‡EÀÛª)Æt„ð¨¢/w}OƒôþRô-/<ÐßïüÒÓ£5BgZ|dêÅYÒ�§)•o¼.@_¤M™jgÒ”?ŒÃ4¹…’Œ?Æ¨eYZY“ãzI6ß¸†�ÆµtÖ= ( Q¢s8 Ò&HÏ;pkþŸ'�;T•ÞÓÞÙ-3â2â7·Øø¯ô&åo‘=´œ©j|‰gSBø=²ßúó›´Ïžã¦|0K;šÂnwwZöü9ßÏÃ& ¬HùƒËÛIÈäc3¯´(+ÄC½ô÷?60'ÙÃ’
ZÓÍ´ŸÃ6�jËB¼�s¸Ö�éì#†×ÝF qïëÉßó¼³ŸÂEË«( }ÑéW3Ê’¡¤¾ÝâH’¸ªv¨8½oðë`¼î?Õû»fa‚:<�îŽêûøO×†rCJƒ…²�‰ŸbètÑ¨[%Å†ûÊ]fMc‡G½
ö7ð#‡Ñ‚§²œîs	s¤¨Ã8#—ãŸekÜ:14£LÂ�Wå6“¨ÂMø“dJRÚÅØ¦ò¹¥1˜KÃ’7EX’œ1tc~Šô.‘rMÙÊù°,,Qóz§gq&UtcWŠYW¾P     ‡-Ýµ�Ïq ñ�Ã %çìW±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  *]<?xml version="1.0"?><Features nextValidID="21"><Feature recentID="0" name="PGAM5" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1566" maxFusedRunOn="1566"><Segment range="706-1470" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLPGVCKVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCSIPPLLSAGDFVLLGS"/></Q></Feature><Feature recentID="1" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="700-709" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6421" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5630-6421" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6422-6490" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="3" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3329-4123" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1587-2175" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4871-5459" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2491-2919" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2933-3262" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3113-3248" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4299-4420" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6491-6595" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2241-2289" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4517-4523" color="#ffffff" type="standard"/><Segment range="4524-4541" color="#ffffff" type="standard"/><Segment name="-35" range="4542-4547" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4562-4583" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4469-4485" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4493-4509" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1566" maxFusedRunOn="1566" detectionMode="exactProteinMatch"><Segment range="1477-1503" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="19" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1566" maxFusedRunOn="1566" detectionMode="exactProteinMatch"><Segment range="1507-1533" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="20" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1566" maxFusedRunOn="1566" detectionMode="exactProteinMatch"><Segment range="1537-1563" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  £<Notes>
<UUID>c8f1c667-dec5-48ac-bf40-ad86a874490a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-PGAM5(human)-3Ã—HA-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="1:8:5">2025.9.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d               E              ÿÿÿÿYô? P                                                                                                                                                             <StrandColors><TopStrand><ColorRange range="698..722" colors="green"/><ColorRange range="1379..1379" colors="magenta"/></TopStrand><BottomStrand><ColorRange range="698..722" colors="green"/><ColorRange range="1379..1379" colors="magenta"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
